Polski
Angielski
The Influence of Calamagrostis epigejos and Solidago gigantea on microbial community structure and activity in the substrate of hard coal spoil heaps
Data: Poniedziałek 24.02.2025
Sesja: Urban-Industrial Novel Ecosystems as a Foundation of the Modern Economy and Development
Godzina/Sala: 10:30 - 10:45 - PIANO
Tytuł: The Influence of Calamagrostis epigejos and Solidago gigantea on microbial community structure and activity in the substrate of hard coal spoil heaps
Title: "The Influence of Calamagrostis epigejos and Solidago gigantea on microbial community structure and activity in the substrate of hard coal spoil heaps"
Streszczenie:
The study focuses on the influence of two plant species commonly present on disturbed sites, native expansive Calamagrostis epigejos and alien invasive Solidago gigantea on microbial community structure and activity. The chosen site is a hard coal spoil heap located in the Southern Poland region (Upper Silesia) To determine the substrate\\\'s microbial activity the ISO/TS 22939 method was used to analyse the enzyme activity and the BIOLOG Ecoplate method was used to determine functional diversity and to analyse the microbial structure the PLFA method was used. To gain a context of the parameters present in the substrate following physicochemical parameters were analysed: pH, electric conductivity, substrate organic matter, total nitrogen content, bioavailable Mg and P as well as exchangeable cations of Ca, Mg, Na, and K. The results determined the influence of the presence of plants as well as the chosen species on microbial communities. The enzyme activity was higher in substrates where plants were present; however, on sites where both species were present, the activity was lower than on sites dominated by one. Additionally, the plots dominated by both C. epigejos and S. gigantea were characterized by higher Gram-negative bacteria biomass and total microbial biomass compared to control plots. In addition, all types of vegetation-covered plot\\\'s parameters were higher than control plots in the biomass of saprophytic fungi and arbuscular mycorrhizal fungi. The biomass of Gram-positive bacteria in the substrate of the plots dominated by S. gigantea was higher than the biomass of these bacteria in the substrate of the control plots, however, the ratios of Gram-positive bacteria to Gram-negative bacteria and bacteria to fungi took the highest value in the control plots. When it comes to the functional diversity, the presence of C. epigejos and S. gigantea, as well as the co-occurrence of these two species in the study plots, resulted in an increase in the functional diversity of microorganisms compared to the control plots; however, simultaneously showed no statistically significant differences between plots with different vegetation types. In conclusion, the study resulted in broadening the knowledge about the influence of these species on microbial communities proving that both of the species have a positive effect on the microbial community structure and activity.
The study focuses on the influence of two plant species commonly present on disturbed sites, native expansive Calamagrostis epigejos and alien invasive Solidago gigantea on microbial community structure and activity. The chosen site is a hard coal spoil heap located in the Southern Poland region (Upper Silesia) To determine the substrate\\\'s microbial activity the ISO/TS 22939 method was used to analyse the enzyme activity and the BIOLOG Ecoplate method was used to determine functional diversity and to analyse the microbial structure the PLFA method was used. To gain a context of the parameters present in the substrate following physicochemical parameters were analysed: pH, electric conductivity, substrate organic matter, total nitrogen content, bioavailable Mg and P as well as exchangeable cations of Ca, Mg, Na, and K. The results determined the influence of the presence of plants as well as the chosen species on microbial communities. The enzyme activity was higher in substrates where plants were present; however, on sites where both species were present, the activity was lower than on sites dominated by one. Additionally, the plots dominated by both C. epigejos and S. gigantea were characterized by higher Gram-negative bacteria biomass and total microbial biomass compared to control plots. In addition, all types of vegetation-covered plot\\\'s parameters were higher than control plots in the biomass of saprophytic fungi and arbuscular mycorrhizal fungi. The biomass of Gram-positive bacteria in the substrate of the plots dominated by S. gigantea was higher than the biomass of these bacteria in the substrate of the control plots, however, the ratios of Gram-positive bacteria to Gram-negative bacteria and bacteria to fungi took the highest value in the control plots. When it comes to the functional diversity, the presence of C. epigejos and S. gigantea, as well as the co-occurrence of these two species in the study plots, resulted in an increase in the functional diversity of microorganisms compared to the control plots; however, simultaneously showed no statistically significant differences between plots with different vegetation types. In conclusion, the study resulted in broadening the knowledge about the influence of these species on microbial communities proving that both of the species have a positive effect on the microbial community structure and activity.
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